Ackles once confirmed that he and Padalecki had in fact became very close and they hold their friendship very dear: We're best friends, period. He's my best friend for life. I don't think it's that remarkable, but it's definitely special. 

In a scene where the stunt doubles are rehearsing a fight, they are Jared and Jensen's actual stunt doubles. In the alternate universe, "Jared" and "Jensen" don't talk to each other. In real life, Jared Padalecki and Jensen Ackles are best friends and were each other's groomsmen.